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Human Cortisol in samples (or standards) competes with a fixed amount of Cortisol on the solid phase supporter for sites on the HRP linked detection antibody specific to Cortisol
Then a biotinylated detection antibody specific for Rat BetaTG/PBP/CXCL7/NAP2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
When the sample containing IL-5 is added to the plate
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL2
The concentration of ANGPTL-4 in unknown samples is determined from the calibration curve which is created by plotting the absorbance values against the standard concentration values
Mouse RANκL (Receptor Activator of Nuclear Factor Kappa B Ligand) ELISA Kit (Mini-Sample) Packaging:96 wells Human Cortisol in samples (orThis Mouse RANL (Receptor Activator of Nuclear Factor Kappa B Ligand) ELISA Kit (Mini Sample) uses the sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to RANL . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for RANL and Avidin Horseradish Peroxidase (HRP) conjugate are added
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