Human RCAN1 (Calcipressin-1) ELISA Kit Packaging:96 wells Standards or samples are added

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MIg

and the color intensity is directly proportional to the amount of Mouse S100A6 present

Substrate solution is then added and color develops in proportion to the amount of Human ABL2

The concentration of Rat ACTH in tested samples can be calculated by comparing the OD of the samples to the standard curve

Then Streptavidin-HRP is added and binds to the Biotinylated PHF7 antibody

Human RCAN1 (Calcipressin-1) ELISA Kit Packaging:96 wells Standards or samples are addedThe Human (RCAN1) Calcipressin 1 ELISA Kit measures Calcipressin 1 in samples. The plate has been pre coated with Human RCAN1 antibody. RCAN1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human RCAN1 Antibody is added and binds to RCAN1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated RCAN1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing