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Then a biotinylated detection antibody specific for Mouse SCF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The plate has been pre-coated with Human AMFR antibody
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CC16
You can calculate the concentration of Human ADAM10 in the samples by comparing the OD of the samples to the standard curve
Substrate solution is then added and color develops in proportion to the amount of Human ATF3
Human ANG-4 (Angiopoietin 4) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Human (ANG 4) Angiopoietin 4 ELISA Kit measures Angiopoietin 4 in samples. The plate has been pre coated with Human ANG 4 antibody. ANG 4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ANG 4 Antibody is added and binds to ANG 4 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated ANG 4 antibody. After incubation unbound Streptavidin HRP is washed away during a washing
