Rat LIF (Leukemia inhibitory factor) ELISA Kit b-R1 Then Streptavidin-HRP is added and

Then Streptavidin-HRP is added and binds to the Biotinylated IRF8 antibody

Calculate the concentration of OTX2 in the sample by plotting standard curve

Each has a molecular weight of approximately 55 KD

Then a biotinylated detection antibody specific for Mouse CCL6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

The concentration of Mouse NFkB2 in the samples is then determined by comparing the OD of the samples to the standard curve

Rat LIF (Leukemia inhibitory factor) ELISA Kit b-R1 Then Streptavidin-HRP is added andThe Rat LIF (Leukemia inhibitory factor) ELISA Kit measures Lif in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Lif antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection