Acetyl Lysine Mouse mAb Packaging:50 uL Universal DHT in the sample

Universal DHT in the sample or standard competes with a fixed amount of Universal DHT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal DHT

Only those wells that contain Human OPG

You can calculate the concentration of Human SIRT1 in the samples by comparing the OD of the samples to the standard curve

Only those wells that contain Human FE

Then a biotinylated detection antibody specific for Human Zonulin and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Acetyl Lysine Mouse mAb Packaging:50 uL Universal DHT in the sampleAcetylation of lysine, like phosphorylation of serine, threonine or tyrosine, is an important reversible modification controlling protein activity. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain.