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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PP
Substrate solution is then added and color develops in proportion to the amount of Rat TNNI2
The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 42 nm
only those wells that contain Mouse PRF1
The concentration of Human HVEM in the samples is then determined by comparing the OD of the samples to the standard curve
Sheep EPO (Erythropoietin) ELISA Kit Packaging:96 wells Standards or samples are addedThe Sheep (EPO) Erythropoietin ELISA Kit measures Erythropoietin in Sheep samples. The plate has been pre coated with Sheep EPO antibody. EPO present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Sheep EPO Antibody is added and binds to EPO in the sample. Then Streptavidin HRP is added and binds to the Biotinylated EPO antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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