Monkey HS-INS (High Sensitivity Insulin) Accquant® ELISA Kit AP-TNAP Then a biotinylated detection antibody

Then a biotinylated detection antibody specific for Human TFR1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit TNFb

The concentration of RETN in the samples is then determined by comparing the OD of the samples to the standard curve

Then a biotinylated detection antibody specific for Human TSP-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

The OD value is proportional to the concentration of Rat NOS2/iNOS

Monkey HS-INS (High Sensitivity Insulin) Accquant® ELISA Kit AP-TNAP Then a biotinylated detection antibodyThis High Sensitivity Insulin ELISA kit is based on Sandwich ELISA detection method and takes approximately 4 hours. The microplate provided in this kit has been precoated with anti INS antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with INS bound to precoated antibody. Wash unbound components and add HRP Streptavidin