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only those wells that contain Human PEBP4
Then a biotinylated detection antibody specific for Mouse IgG2b and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The plate has been pre-coated with Mouse TLR4 antibody
Only those wells that contain Human CCL16
The OD value is proportional to the concentration of Mouse CCL6
Rat NP-W (Neuropeptide W) ELISA Kit Packaging:96 wells only those wells that containThe Rat (NP W) Neuropeptide W ELISA Kit measures Neuropeptide W in Rat samples. The plate has been pre coated with Rat NPW antibody. NPW present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat NPW Antibody is added and binds to NPW in the sample. Then Streptavidin HRP is added and binds to the Biotinylated NPW antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
