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Substrate solution is then added and color develops in proportion to the amount of Mouse MCAM
After the bacteria are broken by lysozyme
a horseradish peroxidase conjugated mouse anti-Human M-CSF Antibody is added to the wells and binds to M-CSF in the sample
And then biotinylated Human GM Antibody is added and binds to GM in the sample
only those wells that contain Mouse GAL1
Human TMOD3 (Tropomodulin 3) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Human (TMOD3) Tropomodulin 3 ELISA Kit measures Tropomodulin 3 in samples. The plate has been pre coated with Human TMOD3 antibody. TMOD3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human TMOD3 Antibody is added and binds to TMOD3 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated TMOD3 antibody. After incubation unbound Streptavidin HRP is washed away during a washing
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