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TNNI2 present in the sample is added and binds to antibodies coated on the wells
Substrate solution is then added and color develops in proportion to the amount of Bovine TGFBR1
Then Streptavidin-HRP is added and binds to the Biotinylated BMP10 antibody
Then a biotinylated detection antibody specific for Human GDF11 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NPY1R
Mouse TSP-1 (Thrombospondin 1) ELISA Kit 15-EPI TNNI2 present in the sampleThe Mouse (TSP 1) Thrombospondin 1 ELISA Kit measures thrombospondin 1 in Mouse samples. The plate has been pre coated with Mouse TSP 1 antibody. TSP 1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse TSP 1 Antibody is added and binds to TSP 1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated TSP 1 antibody. After incubation unbound Streptavidin HRP is washed away during
