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Wash unbound components and add HRP-Streptavidin Conjugate (SABC)
Then Streptavidin-HRP is added and binds to the Biotinylated F7 antibody
ILF3 present in the sample is added and binds to antibodies coated on the wells
RILPL2 present in the sample is added and binds to antibodies coated on the wells
You can calculate the concentration of Rat E-Cad in the samples by comparing the OD of the samples to the standard curve
Human HS-IL-6 (High sensitive Interleukin 6) high sensitive Accquant ELISA Kit TGF-B1 Wash unbound components and addThe Human HS IL 6 (High sensitive Interleukin 6) high sensitive Accquant ELISA Kit measures IL 6 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL 6 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound
