Human RSPO1 (R-Spondin-1) ELISA Kit Packaging:96 wells Substrate solution is then added

Substrate solution is then added and color develops in proportion to the amount of Mouse SP1

Then Streptavidin-HRP is added and binds to the Biotinylated JUNB antibody

The concentration of Human TGFbI in the samples is then determined by comparing the OD of the samples to the standard curve

only those wells that contain Human ITLN2

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MyoD

Human RSPO1 (R-Spondin-1) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Human (RSPO1) R Spondin 1 ELISA Kit measures R Spondin 1 in samples. The plate has been pre coated with Human RSPO1 antibody. RSPO1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human RSPO1 Antibody is added and binds to RSPO1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated RSPO1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.