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Then a biotinylated detection antibody specific for Human TNF-Beta and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The concentration of Mouse FASN in the samples is then determined by comparing the OD of the samples to the standard curve
The OD value is proportional to the concentration of TNFRSF9
Substrate solution is then added and color develops in proportion to the amount of Human DNAJC12
Then Streptavidin-HRP is added and binds to the Biotinylated UBA1 antibody
Rat IKBKB (Inhibitor of Nuclear Factor Kappa-B Kinase Subunit Beta) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Rat (IKBKB) Inhibitor of Nuclear Factor Kappa B Kinase Subunit Beta ELISA Kit measures Inhibitor of Nuclear Factor Kappa B Kinase Subunit Beta in Rat samples. The plate has been pre coated with Rat IKBKB antibody. IKBKB present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat IKBKB Antibody is added and binds to IKBKB in the sample. Then Streptavidin HRP is added and binds to the Biotinylated IKBKB
