Osteopontin (OPN) ELISA SCYB9 and the color intensity is

and the color intensity is directly proportional to the amount of Human LCN1 present

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GCP2

Only those wells that contain Mouse FETUA

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog HGF

You can calculate the concentration of Human VDR in the samples by comparing the OD of the samples to the standard curve

Osteopontin (OPN) ELISA SCYB9 and the color intensity isThe Osteopontin ELISA is an enzyme immunoassay for the quantitative determination of Osteopontin in human serum and plasma. The microtiter plate is coated with the antibody specifically binding the Osteopontin. The human serum or plasma is incubated in the plate with the capture antibody. The specimen is washed out and the specifically bound protein is incubated with biotin labelled detection antibody. Following another washing step, Streptavidin HRP