Human CYB5A (Cytochrome B5) ELISA Kit Packaging:96 wells The concentration of Human APLN

The concentration of Human APLN in tested samples can be calculated by comparing the OD of the samples to the standard curve

the amount of 17-OHP peroxidase conjugate immunologically bound to the well progressively decreases as the concentration of 17-OHP in the specimen increases

Then a biotinylated detection antibody specific for Human SHBG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

You can calculate the concentration of Porcine LH in the samples by comparing the OD of the samples to the standard curve

Mouse D2D in the sample or standard competes with a fixed amount of Mouse D2D on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse D2D

Human CYB5A (Cytochrome B5) ELISA Kit Packaging:96 wells The concentration of Human APLNThe Human (CYB5A) Cytochrome B5 ELISA Kit measures Cytochrome b5 in samples. The plate has been pre coated with Human CYB5A antibody. CYB5A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CYB5A Antibody is added and binds to CYB5A in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CYB5A antibody. After incubation unbound Streptavidin HRP is washed away during a washing