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Substrate solution is then added and color develops in proportion to the amount of Rat AQP2
Then a biotinylated detection antibody specific for Human FGF18 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The concentration of Human SLC6A19 in the samples is then determined by comparing the OD of the samples to the standard curve
The concentration of Human DEFb112 in the samples is then determined by comparing the OD of the samples to the standard curve
The concentration of Mouse SCFR in the samples is then determined by comparing the OD of the samples to the standard curve
Rat IgG (Immunoglobulin G) ELISA Kit SR-PSOX Substrate solution is then addedThe Rat (IgG) Immunoglobulin G ELISA Kit measures Immunoglobulin G in Rat samples. Add samples to the pre coated plate. Then add biotinylated antigen. The antigens in the samples compete with the biotinylated antigen to bind to the capture antibody and incubate. Unbound antigen is washed away during a washing step. An avidin HRP is then added and then incubate. Unbound avidin HRP is washed away during a washing step. TMB Substrate is then added and
