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Substrate solution is then added and color develops in proportion to the amount of Mouse PDHX
The concentration of Human GPR39 in the samples is then determined by comparing the OD of the samples to the standard curve
The OD value is proportional to the concentration of Human PAP
The OD value is proportional to the concentration of Human TLR4
only those wells that contain Human TSPO
Human IL-21 (Interleukin 21) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Human (IL 21) Interleukin 21 ELISA Kit measures Interleukin 21 in samples. The plate has been pre coated with Human IL21 antibody. IL21 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human IL21 Antibody is added and binds to IL21 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated IL21 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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