Bovine IL-12A (Interleukin-12 subunit alpha) ELISA Kit PGRN Human IgG in samples (or

Human IgG in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human IgG

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DEFb126

it binds with LPO bound to precoated antibody

ALB present in the sample is added and binds to antibodies coated on the wells

Then a biotinylated detection antibody specific for Mouse BDNF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Bovine IL-12A (Interleukin-12 subunit alpha) ELISA Kit PGRN Human IgG in samples (orThe Bovine IL 12A (Interleukin 12 subunit alpha) ELISA Kit measures IL 12A in Bovine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL 12A antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add