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The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SIGLEC7
Then a biotinylated detection antibody specific for BDNF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The human serum
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TM
Then Streptavidin-HRP is added and binds to the Biotinylated ADCY6 antibody
Mouse IL-9 (Interleukin 9) ELISA Kit 92kDa Type IV Collagenase The microtiter plate provided inThe Mouse (IL 9) Interleukin 9 ELISA Kit measures Interleukin 9 in Mouse samples. The plate has been pre coated with Mouse IL 9 antibody. IL 9 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse IL 9 Antibody is added and binds to IL 9 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated IL 9 antibody. After incubation unbound Streptavidin HRP is washed away during a washing
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