Rat RUNX2 (Runt-related Transcription Factor 2) ELISA Kit Packaging:96 wells Standards or samples are added

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse FASN

Then a biotinylated detection antibody specific for TIMP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Horseradish peroxidase (HRP) bound in the complex reacts with the chromogenic substrate (TMB) creating the blue colour

a horseradish peroxidase conjugated mouse anti-Human TIMP-1 Antibody is added to the wells and binds to TIMP-1 in the sample

only those wells that contain Sheep IL1b

Rat RUNX2 (Runt-related Transcription Factor 2) ELISA Kit Packaging:96 wells Standards or samples are addedThe Rat (RUNX2) Runt related Transcription Factor 2 ELISA Kit measures Runt related transcription factor 2 in Rat samples. The plate has been pre coated with Rat RUNX2 antibody. RUNX2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat RUNX2 Antibody is added and binds to RUNX2 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated RUNX2 antibody. After incubation unbound