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Substrate solution is then added and color develops in proportion to the amount of Rat FLOT1
a horseradish peroxidase conjugated mouse anti-Human CIB2 Antibody is added to the wells and binds to CIB2 in the sample
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig IL18
And then biotinylated Human CSTA Antibody is added and binds to CSTA in the sample
The assay plate has been pre-coated with mouse anti-Human PD-L1 monoclonal antibody
Rat ERFE (Erythroferrone) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Rat (ERFE) Erythroferrone ELISA Kit measures Erythroferrone in Rat samples. The plate has been pre coated with Rat ERFE antibody. ERFE present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat ERFE Antibody is added and binds to ERFE in the sample. Then Streptavidin HRP is added and binds to the Biotinylated ERFE antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.