Rat MMP-2 (Matrix Metalloproteinase 2) ELISA Kit FIAF Substrate solution is then added

Substrate solution is then added and color develops in proportion to the amount of Human CTRP-1

The concentration of Cattle ADP in the samples is then determined by comparing the OD of the samples to the standard curve

And then biotinylated Human CADM1 Antibody is added and binds to CADM1 in the sample

And then biotinylated Mouse SPP1 Antibody is added and binds to SPP1 in the sample

The Mouse CA19-9 (Carbohydrate Antigen 19-9) ELISA Kit is a Sandwich enzyme immunoassay

Rat MMP-2 (Matrix Metalloproteinase 2) ELISA Kit FIAF Substrate solution is then addedThe Rat (MMP 2) Matrix Metalloproteinase 2 ELISA Kit measures Matrix Metalloproteinase 2 in Rat samples. The plate has been pre coated with Rat MMP2 antibody. MMP2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat MMP2 Antibody is added and binds to MMP2 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated MMP2 antibody. After incubation unbound Streptavidin HRP is washed away