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Substrate solution is then added and color develops in proportion to the amount of Human PLK3
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KRT13
LTB4R2 present in the sample is added and binds to antibodies coated on the wells
Then Streptavidin-HRP is added and binds to the Biotinylated KDR antibody
The concentration of Rat LTb in the samples is then determined by comparing the OD of the samples to the standard curve
Goat IL1B (Interleukin-1 beta) ELISA Kit GELB Substrate solution is then addedThe Goat IL1B (Interleukin 1 beta) ELISA Kit measures IL 1Beta in Goat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL1B antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
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