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When the sample containing BMP-2 is added to the plate
The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 33 nm
Then Streptavidin-HRP is added and binds to the Biotinylated H1-1 antibody
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat VIP
The assay plate has been pre-coated with mouse anti-Human L-selectin monoclonal antibody
Human GSTO1 (Glutathione S-transferase Omega-1) ELISA Kit THPO When the sample containing BMP-2The Human (GSTO1) Glutathione S transferase Omega 1 ELISA Kit measures Glutathione S Transferase Omega 1 in samples. The plate has been pre coated with Human GSTO1 antibody. GSTO1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human GSTO1 Antibody is added and binds to GSTO1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated GSTO1 antibody. After incubation unbound
