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The OD value is proportional to the concentration of LIF
The concentration of Human COL5a1 in the samples is then determined by comparing the OD of the samples to the standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BCL2L11
Mouse β-EP in the sample or standard competes with a fixed amount of Mouse β-EP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse β-EP
Then a biotinylated detection antibody specific for Human IP-10/CXCL10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Bovine CSF3 (Granulocyte Colony-stimulating Factor) ELISA Kit Packaging:96 wells The OD value is proportionalThe Bovine (CSF3) Granulocyte Colony stimulating Factor ELISA Kit measures Granulocyte Colony Stimulating Factor in Bovine samples. The plate has been pre coated with Bovine CSF3 antibody. CSF3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Bovine CSF3 Antibody is added and binds to CSF3 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CSF3 antibody. After incubation unbound
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