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And then biotinylated Mouse EDN3 Antibody is added and binds to EDN3 in the sample
And then biotinylated Human GLRX1 Antibody is added and binds to GLRX1 in the sample
The OD value is proportional to the concentration of Guinea pig DAP6
You can calculate the concentration of Guinea pig PACAP-38 in the samples by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Guinea pig C4BPAlpha and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Human SELP (P-Selectin) ELISA Kit Packaging:48 wells And then biotinylated Mouse EDN3The Human SELP (P Selectin) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Human SELP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SELP and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well
