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The concentration of Rat COL3a1 in the samples is then determined by comparing the OD of the samples to the standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GROa/CXCL1
The OD value is proportional to the concentration of Rat IL-17A
The assay plate has been pre-coated with mouse anti-Human CD300C monoclonal antibody
The OD value is proportional to the concentration of Human GzmB
Human GYPA (Glycophorin A) ELISA Kit Packaging:96 wells The concentration of Rat COL3a1The Human (GYPA) Glycophorin A ELISA Kit measures Glycophorin A in samples. The plate has been pre coated with Human GYPA antibody. GYPA present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human GYPA Antibody is added and binds to GYPA in the sample. Then Streptavidin HRP is added and binds to the Biotinylated GYPA antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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