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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ACH
a horseradish peroxidase conjugated mouse anti-Rhesus CD32A Antibody is added to the wells and binds to CD32A in the sample
The Mouse FXR (Farnesoid X Receptor) ELISA Kit is a Sandwich enzyme immunoassay
You can calculate the concentration of Human HMGB-1 in the samples by comparing the OD of the samples to the standard curve
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TNFSF13
Rat NPHS2 (Podocin) ELISA Kit Packaging:96 wells Standards or samples are addedThe Rat (NPHS2) Podocin ELISA Kit measures Podocin in Rat samples. The plate has been pre coated with Rat NPHS2 antibody. NPHS2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat NPHS2 Antibody is added and binds to NPHS2 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated NPHS2 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate
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