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The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL10
Then a biotinylated detection antibody specific for Rat LEP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Biotinylated Recombinant Human Mesothelin is produced by our Mammalian expression system and the target gene encoding Glu296-Ser598 is expressed with a 6His
coli expression system and the target gene encoding Met1-Asp237 is expressed with a 6His tag at the N-terminus
The OD value is proportional to the concentration of Rat LEP
Human IL-10 (Interleukin 10) ELISA Kit Packaging:48 wells The microtiter plate provided inThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human IL10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human IL10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added,
