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Cells were incubated overnight and then media was changed to DMEM (Sigma-Aldrich) with 10% BCS and 1x GlutaMAX (Thermo Fisher Scientific)
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As an angler your approach should consider factors such as climate, stock levels, time of year, scale and depth of water, characteristics of the lake bed, clarity and movement of the water, all of which are physically in front of you
This ensures reasonable structural integrity for the whole rack
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